{{AFC submission|t||u=Benjamed|ns=118|demo=|ts=20260215221544}}

{{AFC submission|t||u=Benjamed|ns=118|demo=|ts=20260215221544}}

[[File:TimeVault Design.png|alt=|thumb|550x550px|A visualization of TimeVault design for mRNA storage. Poly(A)-binding protein (PABP, green) was fused to the INT domain (PABP-INT, green-yellow) and co-expressed with MVP (blue) under a doxycycline (Dox) inducible promoter, enabling temporal capture of cellular mRNA via PABP’s affinity for poly(A) tails. Figure Adapted from Chao et al., 2026 {{Cite journal |last=Chao |first=Yu-Kai |last2=Wu |first2=Michelle |last3=Gong |first3=Qiyu |last4=Chen |first4=Fei |date=2026-01-15 |title=A genetically encoded device for transcriptome storage in mammalian cells |url=https://www.science.org/doi/10.1126/science.adz9353 |journal=Science |language=en |doi=10.1126/science.adz9353 |issn=0036-8075}} Created with BioRender.com]]

[[File:TimeVault Design.png|alt=|thumb|550x550px|A visualization of TimeVault design for mRNA storage. Poly(A)-binding protein (PABP, green) was fused to the INT domain (PABP-INT, green-yellow) and co-expressed with MVP (blue) under a doxycycline (Dox) inducible promoter, enabling temporal capture of cellular mRNA via PABP’s affinity for poly(A) tails. Figure Adapted from Chao et al., 2026 {{Cite journal |last=Chao |first=Yu-Kai |last2=Wu |first2=Michelle |last3=Gong |first3=Qiyu |last4=Chen |first4=Fei |date=2026-01-15 |title=A genetically encoded device for transcriptome storage in mammalian cells |url=https://www.science.org/doi/10.1126/science.adz9353 |journal=Science |language=en |doi=10.1126/science.adz9353 |issn=0036-8075}} Created with BioRender.com]]

TimeVault is a genetically encoded system that records and stores [[Transcriptome|transcriptomes]] within living mammalian cells for later readout enabling direct linkage between past gene expression states and future cellular behaviors. TimeVault uses engineered [[Vault (organelle)|vault particles]] to capture [[Polyadenylation|polyadenylated]] [[Messenger RNA|mRNA]] with minimal changes, allowing historical transcriptional states to be preserved for up to 7 days within living mammalian cells for future evaluation. This allows researchers to study how cells respond to stress, make fate decisions, and evolve [[Cellular adaptation|resistance to therapies]] over time.

'''TimeVault''' is a genetically encoded system that records and stores [[Transcriptome|transcriptomes]] within living mammalian cells for later readout enabling direct linkage between past gene expression states and future cellular behaviors. TimeVault uses engineered [[Vault (organelle)|vault particles]] to capture [[Polyadenylation|polyadenylated]] [[Messenger RNA|mRNA]] with minimal changes, allowing historical transcriptional states to be preserved for up to 7 days within living mammalian cells for future evaluation. This allows researchers to study how cells respond to stress, make fate decisions, and evolve [[Cellular adaptation|resistance to therapies]] over time.

== Background ==

== Background ==